Exosomal <scp>NNMT</scp> from peritoneum lavage fluid promotes peritoneal metastasis in gastric cancer

Peritoneal metastasis (PM) is the major cause of recurrence in patients with gastric cancer (GC) and associated poor prognosis. The oncogenic role Nicotinamide N-methyltransferase (NNMT) GC has been reported, but secreted NNMT that transported by exosomes remains unknown. In this study, were isolated from or without PM cell line, including GC-114, GC-026, MKN45, SNU-16 cells. contents significantly enhanced compared those PM. Furthermore, levels lines relative to normal human epithelial line GES-1 These data indicate may be involved intercellular communication for peritoneal dissemination. Moreover, colocalization GC-derived exosomal was found mesothelial HMrSV5 Additionally, exosomes, activated TGF-?/smad2 signaling However, when silenced, activation abolished We propose NNMT-containing derived cells could promote via signaling.